ABSTRACT
Objective: To study the chemical constituents in the root of Actinidia chinensis. Methods: The chemical constituents were isolated and purified by silica gel column and their structures were identified on the basis of spectral methods. Results: Eleven compounds were isolated and identified as γ-quinide (1), stearyl-β-D-glucopyranoside (2), daucosterol (3), (-)-epi-catechin (4), 2α,3α,24-trihydr-oxyurs-12-en-28-oic acid (5), 3-epi-corosolic acid (6), ursolic acid (7), n-butyl-β-D-fructopyranoside (8), sucrose (9), lignocericacid (10), and β-steriol (11). Conclusion: Compound 1 is isolated for the first tine as natural substance, and compounds 2,8, and 10 are isolated from A. chinensis for the first time.
ABSTRACT
Aim To evaluate the anti-tumor activity of the extract from the root of Actinidia chinensis planch in vitro and in vivo. Methods Active components from the root of Actinidia chinensis planch were isolated by traditional phytochemical techniques. The in vitro anti-tumor activity was determined by sulforhodamine B assay and the in vivo anti-tumor activity was evaluated using experimental mouse tumor models and human tumor xenografts in nude mice. Results Powdered air-dried roots of Actinidia chinensis planch were percolated with methanol at room temperature thrice. The filtrate was concentrated to dryness in vacuo and then was further extracted with ethyl acetate, n-butanol , and chloroform. The fraction extracted by chloroform displayed the most potent activity against several tumor cell lines including hepatocellular carcinoma Bel-7402 cells, non-small cell lung cancer A549 cells, lymphoma Ramos cells, and breast cancer MCF-7 cells. Further more, the anti-tumor efficacy of the chloroform fraction was confirmed in Bel-7402 xenografts in nude mice with the percentage inhibition of 38.0 %. Conclusion The extract of the root of Actinidia chinensis planch has anti-tumor activity, and the active components are mainly in the fraction extracted by chloroform.
ABSTRACT
The inhibitory effect of Actinidia chinensis Planch(ACP) juice on the endogenous formation of N-nitroso compounds (NOC) in the subjects from a high-risk area for gastric cancer were observed. Total concentration of volatile N-nitrosamines(VNA)in fasting gastric juice and 24-h urinary excretion of N-nitrosoproline (NPRO) were used as indices of endogenous exposure. After iagestion of 30 ml ACP juice the average total VNA concentration was significantly decreased from 2.08?1.06?g/L to 0.42?0.43?g/L (p
ABSTRACT
This paper deals with the inhibitory effects of Actinidia chinensis Planch (AC) on the formation of N-nitrosoproline (NPRO). In self-control studies, 15 male rats and 10 healthy men were the subjects. It was found that AC juice inhibited formation of NPRO in vivo in rats (inhibitory rate, 59.6%), and the effect was better than the same amount of a vitamin C (VC) solution (41.8%); and 150g AC fruit containing 75mg VC could completely inhibit the NPRO formation in vivo in men ingesting 300mg NaNO3 and 500mg L-proline, but 75mg VC only partially inhibited.
ABSTRACT
In order to observe the effect of Actinidia chinensis Planch fruit juice on blocking the formation of N-nitrosamide in vivo, 99 pregnant Wistar rats were divided into five groups. The rats of each group were orally given ni-trosamide precursor ethylurea (0.25, 0.50, 1.00, 2.00 4.00 mmol/kg) and NaNO2 (0.125, 0.25, 0.50, 1.00, 2.00 mmol/kg) respectively once a day for three days, on the 7th, 8th and 9th day of pregnancy. Half of the rats in. each group were administrated two precursors and a 4% starch solution, while the other half were administrated two precursors and the tested fruit juice.In the groups without the juice, NEU was formed in vivo, which led to a high mortality of embryos. The embryolethality was 5.21%, 43.66%, 71.7%, 85.8% and 100% respectively, and 4 pregnant rats died in the highest dosage group. However, the rats who received both the two precursors and the tested fruit juice, the living embryos and embryolethality were similar to the control groups except the highest dosage group. The none treatment group and groups only given one of the precursors were done as control groups. The results suggested that the concentrated juice could block the formation of NEU in vivo and prevent the embryotoxicity of NEU.
ABSTRACT
A model system which simulated the conditions of the human stomach was used in the experiments. Precursors NaNO2 and MNG formed MNNG and resulted in the positive mutagenic response to Salmonella typhimurium TA 100. When the concentration of the precursors was 50 mM NaNO2 and 100 mM MNG, the induced average number of revertant per plate was 4327 showed an approximate twenty fold spontaneous revertants. With diluting of the precursors, the mutagenic response was lower, showing a dose-response relationship between the mutagenic activity and the precursors concentration. It was found that, when precursor concentration was lower than 22.2 mM NaNO2 and 44.4 mM MNG, the formation of MNNG was blocked completely by the Chinese Kiwi fruit juice and mutation was inhibited. In the same concentration without the Chinese Kiwi fruit juice, the induced revertant was an approximate 13 fold of the spontaneous revertant. The Chinese Kiwi fruit could not block the formation of the MNNG when the precursors concentration was higher than 33.3 mM NaNO2, but it inhibited the mutagenic activity partly. Compared with the Chinese Kiwi fruit juice, the effect of the ascorbic acid solution in the same concentration was much less.It was demonstrated with TLC that MNNG was formed in the model system.